Introduction to Spectrophotometry & Beer’s Law

Spectrophotometric Determination of Iron Using 1,10-Phenanthroline
SPECTROSCOPY.
427 PHC. Introduction  Spectrometric methods are a large group of analytical methods that are based on atomic and molecular spectroscopy.  Spectroscopy.
Spectrophotometry Chapter 17, Harris
CHMI E.R. Gauthier, Ph.D. 1 CHMI 2227E Biochemistry I Refresher: - acid-base chemistry - spectrophotometry.
Determining the k a of an acid- base indicator Experiment 3.
Determining the ka of an acid-base indicator
Lecture 30 11/14/05. Spectrophotometry Properties of Light h = x J-s c = 3.00 x 10 8 m/s.
Outline Final Comments on Titrations/Equilibria Titration of Base with a strong acid End-point detection Choice of indicators Titration Curve method Start.
Introduction to Spectrophotometry
B EER ’ S L AW P0P0. U SES OF B EER ’ S L AW - Relates concentration to the optical measurement of ‘absorbance’ - combined with spectrophotometry can.
UV/Visible Spectroscopy
Chapter 2 Quantitative.
Lecture 2b. Electromagnetic Spectrum Visible range: = nm Ultraviolet: = nm Low energyHigh energy.
Scanning spectrophotometry and spectrophotometric determination of concentration BCH 333 [practical]
Scanning spectrophotometry and spectrophotometric determination of concentration BCH 333 [practical]
Introduction to Instrumental Analysis - Spectrophotometry
Determination of Concentration Using Spectrophotometry
Dilution 2003 Required D. Information Given A student is instructed to determine the concentration of a solution of CoCl 2 based on absorption of light.
Let’s Talk About Beer Beer’s Law and Concentrations.
Experiment : Solutions Preparation, Part 2
Oximeter What It Does How It Works Dr. Frank Walmsley.
SPECTROPHOTOMETRY. Determines concentration of a substance in solution by Measures light absorbed by solution at a specific wavelength by using spectrophotometer.
ULTRAVIOLET-VISIBLE SPECTROSCOPY (UV-VIS). UV-VIS Absorbance of energy in the UV-Vis region results in the movement of an electron from the ground state.
Spectrophotometry: An Analytical Tool
Microplate reader spectrophotometer. The Beer-Lambert Law A=abc Now let us look at the Beer-Lambert law and explore it's significance. A is absorbance.
Introduction to Spectrophotometry
EQUILIBRIUM CONSTANT DETERMINATION
More Solutions Stuff! Raoult’s Law (volatile)
  Examining how much light is absorbed by a compound’s sample at various wavelengths  Spectrum peaks—  Indicates the wavelengths associated with electrons’
Spectrophotometer.
SPECTROPHOTOMETRY. Spectrophotometry Determines concentration of a substance in solution –Measures light absorbed by solution at a specific wavelength.
SPECTROPHOTOMETRY. Principle : there is interaction between the light and sample particle, spectrophotometer is employed to measure the amount of light.
Outline Start Chapter 18 Spectroscopy and Quantitative Analysis.
Determination of Concentration Using Spectrophotometry
Lecture 21 UV/Vis Pick up Lecture Problem 7 This Week in Lab: Work on 1st Synthetic Next Week in Lab: Ch 9 Final Report Due Synthetic #2 PreLab Due.
Introduction to Spectrophotometry & Beer’s Law
Photometry.
Scanning spectrophotometry and spectrophotometric determination of concentration BCH 333 [practical]
Absorption Spectroscopy CHEM 251 Week of November 1 st, 2010 Alexis Patanarut.
11 Instrumental Analysis Tutorial By the end of this session the student should be able to: 1.Use mathematical formulae to calculate absorbance,
A darker color means a higher concentration of the colored component
Absorption spectrum and spectrophotometric determination of concentration Experiment 1.
Investigating the relationship between Molar Concentration and Absorbance of Light Beer’s Law Link to spec 20 video.
Theory of Spectrophotometry
Absorption Spectrums 9/18/15.
UV/Visible Spectroscopy
Principles and practice of Spectrophotometer
Colorimetry GT Chemistry 5/13/15.
Beer’s Law P0 Uses of Beer’s Law
Spectrophotometry.
Spectrophotometry For high school Chemistry, AP Chemistry,
Beer’s Law Colorimetry Colligative Properties Review
COLORED SOLUTIONS A solution will appear a certain color if it absorbs its complementary color from the color wheel EX2-1 (of 24)
Lecture 2b Beer’s Lambert Law.
DIPHENYLPOLYENE DYE SPECTRA
Measurement of Analytes

Cu2+ + 4NH3 → Cu(NH3)42+ (deep blue)
Light and Matter Main Concept:
Lab# 1 Scanning spectrophotometry and spectrophotometric determination of concentration BCH 333 [practical]
Let’s Talk About Beer’s Law
STEP 1 – DETERMINE THE WAVELENGTH OF LIGHT TO USE
Beer's- Lambert Law and Standard Curves of concentrations
Cu2+ + 4NH3 → Cu(NH3)42+ (deep blue)
Let’s Talk About Beer’s Law
Sample AP Model Drawing Question
Solution Preparation Experiment
Spectrophotometry A method to determine concentration of a species exploiting the absorption of EMR.